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MiSeq protocol for Zika virus sequencing DataProtocols

MiSeq protocol for Zika virus sequencing

Amplicon-based protocol for Zika virus sequencing on the Illumina MiSeq The protocol can be found here. Figure 1. Sequencing coverage of the Zika virus genome. Zika virus strain PRVABC59 spiked with Hela RNA was PCR amplified using 70 virus-specific primers in two reactions, prepared for sequencing using the Kapa Hyper prep kit, and sequenced using the MiSeq v2 500 cycle…
August 26, 2016
Zika virus pilot (update) Data

Zika virus pilot (update)

We have now received several hundred de-identified clinical samples from the Dominican Republic and Colombia. Unfortunately, they all have extremely low viral lows (Ct values > 38 in the few that are positive), so we’re continuing our technology development. We are following a couple of different strategies: (more…)
July 21, 2016
Roots, Not Parachutes Publications

Roots, Not Parachutes

Infectious disease outbreaks continue to pose challenges to global health and security, prompting reactive countermeasures. Recently, severe outbreaks of Ebola and Zika virus were designated by the World Health Organization as “Public Health Emergencies of International Concern.” Other emerging viral pathogens have warranted similar attention, including virus outbreaks from Lassa, Chikungunya, avian influenza, Nipah, SARS, and MERS. (more…)
June 30, 2016
Zika virus pilot Data

Zika virus pilot

We recently received plasma samples from Zika virus (ZIKV) patients in Colombia. We performed QC on the samples and unfortunately very few had detectable levels of ZIKV by qPCR. We extracted RNA from two of these patient samples (Z184 and Z186), as well as a positive control (seed stock of the Malaysian strain P6-740 passaged once on BHK-21 cells) kindly…
April 5, 2016
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