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Nathan Grubaugh

Zika sequences from Miami mosquitoes

By | Data, News

Data here. Protocol here. Three pools of Aedes aegypti mosquitoes collected in Miami Beach on August 22nd and 23rd, 2016, were found to be infected with Zika virus. Through our collaborators, Scott Michael and Sharon Isern from Florida Gulf Coast University, we recently received samples of these mosquitoes for sequencing using our amplicon-based protocol for MiSeq.

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Zika sequence from local Florida transmission

By | Data, News

Data here. Protocol here. In collaboration with Dr. Diogo Magnani in the Watkins Laboratory in the Dept. of Pathology at the University of Miami’s Miller School of Medicine, we recently received plasma and saliva from two people with Zika virus infections living in the Miami area. Using our amplicon-based approach previously used to sequence Zika virus from travel-related Zika virus cases in…

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MiSeq protocol for Zika virus sequencing

By | Data, Protocols

Amplicon-based protocol for Zika virus sequencing on the Illumina MiSeq The protocol can be found here. Sequencing Zika virus from the ongoing epidemic in the Americas is of critical importance. Data from the viral genome will help us determine how the virus is spreading, changes to viral population sizes, and if the virus is adapting to local conditions.

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