Protocols

Zika virus plaque assay
By: Glenn Oliveira

This is an easy-to-follow protocol for creating a plaque assay for Zika virus using crystal violet for easy counting.

Lassa virus sequencing
By: Matthias Pauthner

This is an easy-to-follow protocol for high-coverage sequencing of Lassa virus S Segments.

MiSeq protocol for PrimalSeq virus sequencing
By: Nate Grubaugh, Karthik Gangavarapu, Glenn Oliveira, Josh Quick, Nick Loman, Kristian Andersen

This is an easy-to-follow protocol (“PrimalSeq”) that uses “PrimalScheme”-based amplicon sequencing of virus genomes directly on the Illumina platform.

MinION protocol for Zika virus sequencing
By: Josh Quick, Nick Loman, Team ZiBRA

This is an easy-to-follow protocol that uses “PrimalScheme”-based amplicon sequencing of Zika virus genomes directly on the MinION platform.