Functional evolution of Zika virus in the Americas

Summary

To assess the functional evolution of ZIKV, we have generated a library of infectious clones that span the recent ZIKV outbreak in the Americas. We have introduced mutations into the the Pariaba_01 strain to represent the 10 major American ZIKV clades which are defined by amino acid changes. We've also included three clades (also defined by amino acid changes) which pre-date the American outbreak. We will use replicative and competitive fitness assays in mosquitoes and various cell lines to map the phenotypes of these clones.

Phylogenetic tree of ZIKV samples in Asia and the Americas

ZIKV Tree

Zika virus Infectious Clones

CladeZIKV Genome LocusGeneAA locationAncestor AAMutant AAComments
AM-A3534NS1349MV
AM-B (Paraiba_01) No Changes
AM-C8631NS5322IV
10301NS5878DE
AM-D8631NS5322IV
10090NS5808TI
10301NS5878DE
AM-E3534NS1349MV
2788NS1100GA
6327NS3572ML
9240NS5525RC
AM-F3534NS1349MV
428C107DE
AM-G3534NS1349MV
428C107DE
3459NS1324RW
10164NS5833TA
AM-H345C80IT
3534NS1349MV
AM-I345C80IT
3534NS1349MV
8467NS5267VA
AM-J3534NS1349MV
2788NS1100GA
6327NS3572ML
4731NS340VI
9240NS5525RC
Pre-AM-13534NS1349MV
8007NS5114MV
Pre-AM-23534NS1349MV
523prM17SN
6363NS3584YH
8007NS5114MV
Pre-AM-33534NS1349MV
8007NS5114MV
523prM17SN
6363NS3584YH
3070NS1194VA

Disclaimer. Please note that this data is still based on work in progress and should be considered preliminary. If you intend to include any of these data in publications, please let us know – otherwise please feel free to download and use without restrictions. We have shared this data with the hope that people will download and use it, as well as scrutinize it so we can improve our methods and analyses. Please contact us if you have any questions or comments – we’ll buy beers for #ResearchParasites that spot flaws and faults in the data and come up with improvements!


Andersen Lab
The Scripps Research Institute
La Jolla, CA, USA
data@andersen-lab.com

People

GitHub Commits

Data

Collaborators

Nate Grubaugh
Yale School of Public Health

Greg Ebel
Colorado State University

Funding

niaid