Updated protocol for sequencing SARS-CoV-2 using a shorter amplicon scheme for PrimalSeq. The average length of amplicons in this protocol is ~250 nt, making this well-suited to sequencing on NovaSeq, iSeq, and NextSeq platforms.
Protocol for sequencing SARS-CoV-2 using ARTIC PrimalSeq. The average length of amplicons in this protocol is ~400 nt, making this well-suited to sequencing on the MiSeq.
This is an easy-to-follow protocol (“PrimalSeq”) that uses “PrimalScheme”-based amplicon sequencing of virus genomes directly on the Illumina platform. In this updated version, we use spike-ins to control for cross-sample contamination.
Protocol for COVID-19 screening using CDC and WHO primers.
This is an easy-to-follow protocol for creating a plaque assay for Zika virus using crystal violet for easy counting.
This is an easy-to-follow protocol for high-coverage sequencing of Lassa virus S Segments.
This is an easy-to-follow protocol that uses “PrimalScheme”-based amplicon sequencing of Zika virus genomes directly on the MinION platform.
An Amplicon-Based Sequencing Framework For Accurately Measuring Intrahost Virus Diversity Using PrimalSeq And iVar
Genome Biology 20(8) 1-19 (2019)
Protocol Multiplex PCR Method For MinION And Illumina Sequencing Of Zika And Other Virus Genomes Directly From Clinical Samples
Nature Protocols 12(6):1261-1276 (2017)
Enhanced Methods For Unbiased Deep Sequencing Of Lassa And Ebola RNA Viruses In Clinical And Biological Samples
Genome Biology 15(11):519 (2014)